植物研究2011，Vol.31Issue(3)：271-276,6.
桔梗花粉萌发与花粉管生长研究
Pollen Germination and Growth of Platycodon grandiflorum(Jacq.)A.DC
摘要
Abstract
Two-year-old Platycodon grandifiorum( Jacq. ) A. DC trees grown in the experiment field of Xiangju Pharmacy Limited Company were used as the materials. The effects of culture medium, sucrose, PEG, pH value, culture time and temperature on the pollen germination and tube growth of P. grandifiorum were investigated using the method of liquid culture. The results showed that: ( 1 ) the concentration of 100 - 150 g · L-1 PEG could accelerate pollen germination and the growth of pollen tube, the range of 200 - 250 g · L - 1 PEG could remarkably accelerate pollen germination, however, its function on the growth of pollen tube was not distinct. (2)100 g · L-1 sucrose was helpful for the pollens to germinate and grow, however, high concentration of sucrose can would strongly inhibit pollen germination and the growth of pollen tubes. ( 3 ) the optimum culture medium for pollen germination and growth of Platycodon were ME3 + BK + 10％ sucrose + 150 g · L-1 PEG (pH 5.8). (4)pollen germination was better at 25 -40℃, and it was best at 30℃ for 1.5 h.关键词
桔梗/花粉/离体萌发/花粉管生长Key words
Platycodon grandifiorum/ pollen/ germination In vitro/ growth of pollen tubes分类
农业科技引用本文复制引用
刘自刚,呼天明,杨亚丽,佘小平,汪玉琳,薛粉丽,刘凤霞..桔梗花粉萌发与花粉管生长研究[J].植物研究,2011,31(3):271-276,6.基金项目
陕西省科学技术发展计划项目(2008k16-02) （2008k16-02）
陕西省教育厅专项科研计划项目(2010JK525) （2010JK525）
