植物研究2011，Vol.31Issue(3)：300-305,6.
南蛇藤原生质体培养及植株再生
Protoplast Culture and Plant Regeneration of Celastrus orbiculatus Thunb.
摘要
Abstract
Experimental raw material of protoplast was obtained from embryo callus induction of Celastrus orbiculatus Thunb.under the condition of lower temperature at 4℃ and dark treatment for 24 h.The created culture system of protoplast originated from seedlings differentiation of callus regeneration after the culture of settling the shallow-layer solution and solid-liquid double layer culture as well as agarose-embedding based on MS medium.The results showed that: the lower temperature and dark treatment was beneficial to obtain protoplast with high yield and top quality; the optimum combination for the enzyme activity would be: 0.5％ Cellulase +0.5％ Pectinase +5 mg · L-1 MES; the optimum time of enzymatic hydrolysis was 12 h; the optimum mannitol concentration was 13％; moreover, the optimum mode of enzymatic hydrolysis would be: standing for 12 h and oscillating for 0.5 h; meanwhile, better protoplast culture efficiency was based on the culture of settling the shallow-layer solution; the optimum differentiation medium of callus was MS + 6-BA 2.0 mg · L- 1 + IBA 0.1 mg · L- 1 and rooting medium could be 1/2 MS + NAA 0.1 mg· L-1.关键词
南蛇藤/愈伤组织/原生质体培养/植株再生Key words
Celastrus orbiculatus Thunb./ callus/ protoplast culture/ plant regeneration分类
农业科技引用本文复制引用
范小峰,李东波,刘灵霞,刘秀丽,杨颖丽..南蛇藤原生质体培养及植株再生[J].植物研究,2011,31(3):300-305,6.基金项目
国家自然科学基金(30960064) （30960064）
甘肃省自然科学研究基金(1010RJZA027) （1010RJZA027）
甘肃省教育厅研究生导师科研项目(0810-02) （0810-02）
甘肃省庆阳市科技攻关计划项目(1003NKCM067) （1003NKCM067）
