植物研究2011，Vol.31Issue(3)：371-376,6.
响应面法优化酶解辅助提取翅果油树叶总黄酮
Optimization of Enzyme-assisted Extraction of Total Flavonoids from Elaeagnus mollis Diels Leaves by Using Response Surface Methodology
摘要
Abstract
The process of enzyme-assisted extraction of total flavonoids from the leaves of Elaeagnus mollis Diels was optimized.Based on the single-factor tests, the effects of enzyme amount, time of enzymatic hydrolysis,pH value and temperature of enzymatic hydrolysis on the extraction yields of total flavonoids from E.mollis Diels leaves were studied by response surface methodology (RSM).The results indicated that cellulase showed better ability of enzymatic hydrolysis.The optimum enzyme-assisted extraction process was: enzyme amount 6.5 mg· g-1, time 1.5 h, pH value 4.7 and temperature 46℃.The highest yield of total flavonoids was 7.1％.关键词
翅果油树叶/总黄酮/酶解/响应面Key words
Elaeagnus mollis leaves/ total flavonoids/ enzymatic hydrolysis/ RSM分类
农业科技引用本文复制引用
郭彩珍,薛丹,侯学敏,张直峰,闫桂琴..响应面法优化酶解辅助提取翅果油树叶总黄酮[J].植物研究,2011,31(3):371-376,6.基金项目
国家自然科学基金项目(30470296) （30470296）
山西省自然科学基金项目(2008011058-1) （2008011058-1）
