植物研究2011，Vol.31Issue(3)：381-384,4.
多西紫杉醇白蛋白微球的制备及优化实验研究
Preparation and Optimization Experiment of Docetaxel-loaded Bovine Serum Albumin Nanoparticles
张智舟 1姜守刚 1祖元刚 1赵冬梅 1赵修华 1金晓慧1
作者信息
- 1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨,150040
- 折叠
摘要
Abstract
Docetaxel ( DT), the only derivative of paclitaxel for clinical application in tumor, is mainly used for the treatment of breast cancer, nonsmall-cell lung cancer, oophoroma, etc. Because of its low solubility, organic solvents and solution adjuvants are always added to the preparation. However, organic solvents and solution adjuvants are pungent. Solubility of docetaxel can be increased by the preparation of docetaxel-loaded bovine serum albumin nanoparticles (DT-BSA-NPs) through desolvation-chemical crosslinking method. In this study, the important factors during preparation were tested, and the optimal conditions were obtained according to the optimized data by Design-expert. These data were as follows: the concentration of albumin was 35 mg · mL-1 , the concentration of DT was 1.03 mg · mL-1, the ratio of ethanol to water was 3:1, the dripping speed of ethanol was 0.73 mL · min-1, the stirring time was 12 h, cross-link ratio of glutaraldehyde and BSA was 2:1. The particle size of the product is 185 nm, and the drug-loading rate is 14.4％. The developed method successfully helped to improve the water-solubility of docetaxel and therefore provided a good foundation for the animal experiments and clinical applications in days to come.关键词
多西紫杉醇/白蛋白微球/去溶剂化-化学交联法Key words
docetaxel/ BSA-NPs/ desolvation-chemical crosslinking method分类
农业科技引用本文复制引用
张智舟,姜守刚,祖元刚,赵冬梅,赵修华,金晓慧..多西紫杉醇白蛋白微球的制备及优化实验研究[J].植物研究,2011,31(3):381-384,4.
