动物营养学报2011，Vol.23Issue(6)：1006-1010,5.DOI:10.3969/j.issn.1006-267X.2011.06.018
微生物酶法测定菜籽粕中硫甙含量
Determination of the Glucosinolate Content in Rapeseed Meal Using a Microbial Enzyme Method
摘要
Abstract
Rapeseed meal is an excellent resource of vegetable protein, whose detoxication and application have acquired extensive attention. Aspergillas niger is one of the microbes to detoxicate rapeseed meal. It can decrease the contents of phytin acid and glucosinolate in rapeseed meal effectively. The aim of the study is to establish an analytical method for the determination of glucosinolate in rapeseed meal with myrosinase. In this study, the myrosinase is extracted from selected Aspergillus niger H23 with glucosinolate-decomposing function via ultrasonication. It is the first time that microbial enzymes are used in the quantitative determination of glucosinolate. The process is simple and standard. The stability analysis shows that the method is much more stable than that of the endogenous myrosinase method. The study provides a new concept of glucosinolate analysis and a new way for the application of microbial myrosinase. [ Chinese Journal of Animal Nutrition, 2011, 23(6) :1006-1010]关键词
黑曲霉/微生物酶/硫甙/超声破碎/3,5-二硝基水杨酸Key words
Aspergillus niger/ microbial enzyme/ glucosinolate/ ultrasonication/ 3,5-dinitrosalicylic acid分类
农业科技引用本文复制引用
雷蕾,高雪华,邓小晨..微生物酶法测定菜籽粕中硫甙含量[J].动物营养学报,2011,23(6):1006-1010,5.基金项目
四川省科技厅资助项目(07SG111-8) （07SG111-8）
