华西口腔医学杂志2011，Vol.29Issue(3)：229-232,4.DOI:10.3969/j.issn.1000-1182.2011.03.002
三维培养模型中胰岛素样生长因子-I对人牙周膜细胞增殖及碱性磷酸酶活性的影响
The effect of insulin-like growth factor-Ⅰ on the proliferation and alkaline phosphatase activity of humanperiodontal ligament cells under thrce-dimensional culture system
摘要
Abstract
Objective To investigate the effect of insulin-like growth factor- Ⅰ (IGF- Ⅰ ) on the proliferation and alkaline phosphatase (ALP) activity of human periodontal ligament cells (hPDLCs) under three-dimensional (3D) culture system.Methods The hPDLCs were cultured from periodontium of human teeth by the outgrowth method.Rotary cell culture system(RCCS) was enrolled to set 3D culture system.Samples were set to four groups: Negative control group, positive control group(3D group, IGF-Ⅰ group), and experimental group(3D with IGF-Ⅰ group).Proliferation was tested with methylthiazolyl tetrazolium(MTT), and ALP activity was assayed by spectrophotometer at 1, 3, 5, 7d respectively.Results Compared with that of negative control group, cell proliferation increased significantly in 3D with IGF- Ⅰ group since 3d (P＜0.05).Besides, the cell proliferation of 3D with IGF- Ⅰ group was significantly higher than that of 3D group (P＜0.05).ALP activity of 3D with IGF- Ⅰ group was significantly higher than that of negative control group, and 3D group at 3, 5, 7 d (P＜0.05).Conclusion IGF-Ⅰ significantly promotes the proliferation and ALP activity of hPDLCs under 3D culture system.关键词
人牙周膜细胞/胰岛素样生长因子-I/增殖/碱性磷酸酶/三维培养Key words
human periodontal ligament cells/ insulin-like growth factor- Ⅰ/ proliferation/ alkaline phosphatase/three-dimensional culture分类
医药卫生引用本文复制引用
李彦,牛忠英,汤楚华,包博,师天鹏,司少艳..三维培养模型中胰岛素样生长因子-I对人牙周膜细胞增殖及碱性磷酸酶活性的影响[J].华西口腔医学杂志,2011,29(3):229-232,4.基金项目
国家自然科学基金资助项目(30870598) （30870598）
