重庆医学2011，Vol.40Issue(23)：2341-2342,2345,后插二,4.DOI:10.3969/j.issn.1671-8348.2011.23.020
两种不同肝纤维化相关细胞因子shRNA重组质粒的构建
Construction of two different of hepatic fibrosis-related cytokines shRNA recombinant plasmid
摘要
Abstract
Objective To construct short hairpin RNA (shRNA ) expression plasmid targeting at genes of rat connective tissue growth factor (CTGF) or tissue inhibitor of metalloproteinase 1 (TIMP1).Methods According to the most effective RNA interference sequences of rat CTGF gene and TIMP-1 gene screened out in the previous experiments, two pairs of CTGF and TIMPlgenespecific shRNA were designed and synthesized.After primer annealing, they were inserted into plasmid psiRNAh7SKGF-Pzeo and named as psiRNA-GFP-CTGF or psiRNA-GFP-CTGF, respectively.The two recombinant plasmids were confirmed by restriction enzyme digestion and sequencing.Results Restriction enzyme digestion and sequencing showed that the two doublestranded DNA fragments were inserted correctly into psiRNA-h7SKGFPzeo vectors as expected, respectively.Conclusion Two shRNA recombinant plasmids were constructed successfully, this would establish an experimental foundation for further exploring of new ways in gene therapy for hepatic fibrosis.关键词
金属蛋白酶类/RNA干扰/质粒/肝纤维化/生长因子Key words
metalloproteases / RNA interference / plasmid / hepatic fibrosis / growth factor引用本文复制引用
蒋玉凤,刘加群,张建军,孙华丽,黄飞..两种不同肝纤维化相关细胞因子shRNA重组质粒的构建[J].重庆医学,2011,40(23):2341-2342,2345,后插二,4.基金项目
四川省卫生厅科技项目(080197) （080197）
四川省重点学科资助项目(SZD 0421). （SZD 0421）
