中国脑血管病杂志2011，Vol.8Issue(5)：253-258,6.DOI:10.3969/j.issn.1672-5921.2011.05.007
甘露醇预处理后骨髓问充质干细胞移植对血管性痴呆大鼠认知功能及脑源性神经生长因子表达的影响
Effect of mannitol combined with bone marrow mesenchymal stem ceils on cognitive function and the expression of brain-derived neurotrophic factor of vascular dementia rats
摘要
Abstract
Objective To investigate the effect of mannitol combined with the vein graft supported with bone marrow mesenchymal stem cell ( BMSC ) on the cognitive function and the expression of brain-derived neurotrophic factor ( BDNF ) in brain tissue of vascular dementia ( VD ) rats.Methods The BMSCs of rat were isolated and purified by whole bone marrow culture and adherence screening methods.Seventy nine healthy male SD rats were randomly assigned to sham operation group ( n = 10 ) and model ( n = 69) groups.A VD model was established by the ligation of the bilateral common carotid arteries at an interval of 3 days.Four weeks after modeling, the 32 rats in the model group were randomly allocated into mannitol pretreatment BMSC transplantation group( tail intravenous injection of mannitol 1.5 g/kg, tail intravenous injection of 1 × l06 mL BMSCs 1 mL after pretreatment for 1 to 30 minutes), BMSC transplantation group( injecting the same amount of BMSCs but not mannitol), and medium control group( injecting the same amount of basal medium).After removing the dead rats, there were 9, 11, and 8 rats, respectively in the above 3 groups.Seven rats were included in the sham operation group.Morris water maze test was used to detect cognitive function of the rats, and an enzyme-linked immunosorbent assay was used to detect the BDNF content in brain tissue 4 weeks after intervention.Results ①Comparing the 4 groups at the same time points, the escape latency in the BMSC transplantation group was shorter than that in the medium control group ( all P ＜ 0.01 ), and residence time in the platform quadrant was prolonged ( P ＜ 0.05).The BMSC transplantation group after mannitol pretreatment was also significantly shorter than the escape latency in the BMSC transplantation group (P ＜ 0.01 ), the residence time in the platform quadrant was prolonged ( P ＜ 0.05 ) , and they were all close to the level of the sham operation group ( P ＞ 0.05 ).②The BDNF content in the BMSC transplantation group was significantly higher than that in the medium control group in the frontal cortical and hippocampal regions (P ＜ 0.01 ).The mannitol pretreatment BMSC transplantation group was more significantly higher than the BMSC group (P ＜ 0.01 ) , however, they were lower than the level in the sham operation group (P ＜ 0.01 ).Conclusion Compared with intravenous injection of BMSCs alone, the improvement of cognitive function of the VD rat model treated with mannitol combined with BMSC transplantation is much better; the BDNF expression in cortex and hippocampus is higher.关键词
痴呆,血管性/甘露醇/骨髓细胞/认知/脑源性神经生长因子/大鼠Key words
Dementia/ vascular/ Mannitol/ Bone marrow cells/ Cognition/ Brain-derived neurotrophic factor/ Rats引用本文复制引用
刘金萍,莫雪安,杨龙秀,张泰鹏,陈志..甘露醇预处理后骨髓问充质干细胞移植对血管性痴呆大鼠认知功能及脑源性神经生长因子表达的影响[J].中国脑血管病杂志,2011,8(5):253-258,6.基金项目
广西科学研究与技术开发计划课题(桂科攻0632007-1C) （桂科攻0632007-1C）
