应用化学2011，Vol.28Issue(5)：531-536,6.DOI:10.3724/SP.J.1095.2011.00418
罗丹明B分子印迹聚合物微球的合成及其在固相萃取中的应用
Preparation of Rhodamine B Molecularly Imprinted Polymer Microspheres and Its Application in Solid Phase Extraction
摘要
Abstract
Molecular imprinting technology was employed to produce one kind of rhodamine B ( RhB ) molecularly imprinted polymer (MIP) microspheres by precipitation polymerization using RhB, acrylamide, ethylene glycol dimethacrylatea ( EGDMA ) , azobisisobutyronitrile ( AIBN ) , and acetonitrile as template, functional monomer, cross-linking agent, initiator and porogen, respectively. The resultant microspheres were characterized by scanning electron microscopy. The binding constant and chemical combination ratio of the complex formed between the template and the functional monomer were obtained by UV spectrophotometry, which showed that the RhB-monomer forms 1∶1 complexes and the binding constant is 5. 3 × 103 L/mol. The effects of types and volumes of the porogen on the size and the uniformity of microspheres were also discussed. After packing the microspheres into column, it was used to absorb RhB from red pepper. The extraction conditions of molecular imprinting solid phase extraction ( MISPE) column for RhB were optimized. Pepper samples spiked with RhB were extracted by MISPE column and analyzed by high performance liquid chromatography. The recoveries of MISPE column for RhB extraction were found to be 91.7％～ 103.5％ , indicating the feasibility of the prepared MIPs for RhB extraction.关键词
分子印迹聚合物微球/罗丹明B/沉淀聚合/固相萃取Key words
molecularly imprinted polymer microspheres, rhodamine B, precipitation polymerization, solid phase extraction分类
化学化工引用本文复制引用
雷秀兰,易翔,袁洋,曾延波,李蕾..罗丹明B分子印迹聚合物微球的合成及其在固相萃取中的应用[J].应用化学,2011,28(5):531-536,6.基金项目
浙江省科技计划重点(2007C23071)、嘉兴市科技计划(2010AYl081)资助项目 （2007C23071）
