中国农业科学2011，Vol.44Issue(11)：2225-2233,9.DOI:10.3864/j.issn.0578-1752.2011.11.003
烟草中NAC类转录因子基因的克隆及分析
Cloning and Analysis of NAC Transcription Factor in Tobacco (Nicotiana tabacum L.)
摘要
Abstract
[ Objective ] Cloning and characterization of tobacco NAC transcription factor gene could provide a foundation for studying its function and relationship between the root development and nicotine biosynthesis after tobacco topping. [ Method ] The full-length cDNA sequence of NtNAC-R1 was amplified by in silico cloning and RT-PCR, and was expressed in Escherichia coli BL21. The expression pattern of NtNAC-R1 in root before and after tobacco topping was analyzed by RT-PCR and Northern blotting.[Result] NtNAC-R1 had an 936 bp open reading frame in length, encoding 311 amino acids, with a typical conserved domain of NAC transcription factor family. Phylogenetic analysis showed that NtNAC-R1 had the highest homology with the NAC domain in Petunia and belonged to special NAC family in Solanaceae. This novel gene showed expression activity in prokaryotic cells.NtNAC-R1 had a highest expression level in root, with a decreased expression level at 2 h and 4 h after topping, then rose.[Conclusion] NtNAC-R1 was isolated from tobacco root, and it showed higher transcript levels in the roots, and significantly decreased after 2-4 h of tobacco topping, indicating the NtNAC-R1 can play an important role in the signal transduction of tobacco topping.关键词
烟草/NAC类转录因子/RT-PCR/Northern杂交/组织表达Key words
tobacco (Nicotiana tabacum L.)/ NAC transcription factor/ RT-PCR/ Northern blotting/ tissue expression引用本文复制引用
戚元成,王菲菲,刘卫群,高美娟..烟草中NAC类转录因子基因的克隆及分析[J].中国农业科学,2011,44(11):2225-2233,9.基金项目
国家自然科学基金(30971704) （30971704）
