中国农业科学2011，Vol.44Issue(11)：2347-2354,8.DOI:10.3864/j.issn.0578-1752.2011.11.018
FSH和17β-雌二醇联合作用对仔猪睾丸支持细胞Skp2表达的调节
Effect of Combination of FSH and 17β-estradiol on the Expression of Skp2 in Cultured Immature Boar Sertoli Cell
摘要
Abstract
[Objective] The objective of the study was to identify whether the combination of FSH and 17β-estradiol regulates the expression of S-Phase kinase-associated proteins2 (Skp2) in cultured immature boar sertoli cell via extracellular signal-regulated kinase1/2 (ERK1/2). [Method] The cultured immature boar sertoli cells were treated with the combination of 17βestradiol (10-9 mol·L-1) and FSH (50 ng·mL-1), in addition to a variety of signaling pathway inhibitors and Western blotting, Real-time PCR was applied to detect the expression of Skp2 protein and mRNA, respectively. [ Result ] Treatment with combined FSH and 17β-estradiol increased the concentration and mRNA abundance of Skp2 from 15 mins to 90 mins, and these activities peaked at 30 mins in comparison to the controls (P＜0.05). FSH (50 ng·mL-1), 17β-estradiol (10-9 mol·L-1) and forskolin alone increased the amount of Skp2 protein and the abundance of Skp2 mRNA (P≥0.05 for ail). Rp-cAMP (cAMP inhibitor), H-89 (protein kinase A inhibitor),verapamil (L-type Ca2+ ionic channel inhibitor) and U0126 (ERK1/2 inhibitor) impaired the effect of combined FSH and 17β-estradiol on the expression of Skp2 in both protein and mRNA levels (P＜O.05), however, no significant effects were found on the expression of Skp2 in both protein and mRNA levels when they were tested alone. Likewise, there was no significant effects on activity of ERK1/2 by using H-89 and verapamil alone, but the activity of ERK1/2 was impacted by combination of FSH 17β-estradiol, H-89 and verapamil (P＜ 0.05). [ Conclusion ] cAMP-PKCA pathway and Ca2+influx are activated by the combination of FSH and 17β-estradiol, and both of them are associated with ERK1/2 activation, which regulates the expression of Skp2.关键词
FSH/17β-雌二醇/睾丸支持细胞/S期激酶相关蛋白2/细胞外信号调节的蛋白激酶1/2Key words
FSH/ 17β-estradiol/ sertoli cell/ S-phase kinase-associated proteius2/ extracellular signal-regulated kinase1/2引用本文复制引用
张国升,左敬,甘瑞,朱峰伟,孙燕,王鲜忠,张家骅..FSH和17β-雌二醇联合作用对仔猪睾丸支持细胞Skp2表达的调节[J].中国农业科学,2011,44(11):2347-2354,8.基金项目
国家自然科学基金项目(31072183)、中央高校基本科研业务费专项项目(XDJK2009B035) （31072183）
