日用化学工业2011，Vol.41Issue(3)：232-234,3.
反相高效液相色谱法测定化妆品中巯基乙酸
Determination of thioglycolic acid in cosmetics by reversed- phase high performance liquid chromatography
摘要
Abstract
A reversed -phase high performance liquid chromatographic method was developed for the determination of thioglycolic acid (TGA) in cosmetics. Chromatography separation was carried out on a Kromasil C18 column. Mobile phase for isocratic elution was a blend solution consists of acetonitrile and 0. 01 mol/L potassium dihydrogen phosphate solution (pH adjusted to 2. 5 by phosphoric acid) with volume ratio of 10: 90, and flow rate of 1.0 mL/min. Detection was carried out at 215 nm with column temperature maintained at 30 ℃. The sample injected was 20 μL. Results showed that under those conditions, when the mass concentration of thioglycolic acid ranges within 5.0 ～ 150. 0 μg/mL,it indicates good linear relationship against the peak area. The linearity regression equation can be expressed as Y = 5 100X - 13 400 (r =0. 999 8). The average recoveries achieve ranging from 98.4％ to 100. 8％ with relative standard deviations of 0. 09％ ～ 0. 84％.关键词
化妆品/巯基乙酸/反相高效液相色谱法Key words
cosmetics/ thioglycolic acid/ reversed - phase high performance liquid chromatography分类
化学化工引用本文复制引用
张鹏祥,穆旻 ,刘洋,赵华..反相高效液相色谱法测定化妆品中巯基乙酸[J].日用化学工业,2011,41(3):232-234,3.基金项目
国家食品药品监督管理局专项资助项目[2010] （）
