棉花学报2011，Vol.23Issue(3)：219-223,5.
转phyA基因棉花纯合株系筛选及其对土壤有机磷的利用能力
Identification of Homogeneous Transgenic Lines with phyA Gene in Cotton and Its Utilization for Organic Phosphate in Soil
摘要
Abstract
To obtain homogeneous transgenic lines with expression of phyA and to identify the organic phosphate utilization ability of the transgenic cotton plants, PCR amplification, secreted phytase activity, the available phosphorus content and the yield characters oftransgenic cotton plants were analyzed. The results showed that two(L6 and L9) out of the four lines were homogeneous lines by PCR amplification. Under the phytate condition, the phytase activity of transgenic lines was higher than all of the wide types, and L6 increased by 1.5-fold. Also, L6 kept best performance in phosphorus content with an increase of 4.5％,5.95％, 5.45％, and 8.73％ at seedling, budding, blooming and boll-opening stage, respectively. Moreover, the number of bolls per plant, lint index and lint percentage increased in the transgenic plants. Therefore, it is clearly shown that phyA gene with high expression can significantly increase the ability of cotton to utilize organic phosphate.关键词
棉花(Gossypium hirsutum L.)/植酸酶基因(phyA)/转基因植株/有机态磷Key words
cotton (Gossypium hirsutum L.)/ phytase gene (phyA)/ transgenic plants/ organic phosphate分类
农业科技引用本文复制引用
黄惠,王省芬,郭彩菊,刘建凤,王敬敬,李志坤,吴立强,张桂寅,马峙英..转phyA基因棉花纯合株系筛选及其对土壤有机磷的利用能力[J].棉花学报,2011,23(3):219-223,5.基金项目
农业部重大专项(2009ZX08005-021B) （2009ZX08005-021B）
