棉花学报2011，Vol.23Issue(3)：224-227,4.
转基因抗虫棉中cry1Aa基因表达盒的序列测定和检测
The Sequence and Detection of the cry1Aa Gene Expression Cassette in Transgenic Bt Cotton
摘要
Abstract
A new Bt gene expression cassette cry1Aa gene expression cassette was identified from transgenic insect-resistance cotton "Nannong 6 F1" in this study. The difference was found located at the connection region between Bt gene and 7S UTR terminator while being compared with the crylAc gene and cry1Ab/Ac gene expression cassettes reported previous. Primer pair cry1Aa-F/crylAa-R was designed and construct-specific detection method of cry1Ab/Aa gene expression cassette was established based on the difference. Eight of eighteen cotton varieties from Jiangsu province were identified to have crylAa gene expression cassette using this methods. The method established was useful for identification Bt gene expression cassette and analysis oftransgenic lineages.关键词
转基因棉花/cry1Aa基因/表达盒/PCR检测Key words
transgenic cotton/ cry1Aa gene/ expression cassette/ PCR detection分类
农业科技引用本文复制引用
孙爻,谢家建,范荫荫,李蕊,彭于发..转基因抗虫棉中cry1Aa基因表达盒的序列测定和检测[J].棉花学报,2011,23(3):224-227,4.基金项目
国家重大基础研究发展规划项目(2007CB109201),转基因新品种培育重大科技项目(2011ZX08012-002) （2007CB109201）
