棉花学报2011，Vol.23Issue(3)：259-264,6.
新陆早32号、33号的体细胞胚胎发生和植株再生比较研究
Comparative Study of Somatic Embryogenesis and Plant Regeneration in Cotton Cultivars from Xinluzao 32 and Xinluzao 33(Gossypium hirsutum L.)
摘要
Abstract
An efficient somatic embryo procedure was developed to regenerate plantlets from hypocotyls of cotton cultivars Xinluzao 32 and Xinluzao 33. Calli were effectively produced on the medium with IBA/KT hormone regime, but calli were browned and went to death gradually during the process of proliferation. Embryonic calli of Xinluzao 33 appeared earlier on media with 0.1 mg·L-12,4-D+0.1 mg·L-1 KT hormone combination than on other media. Embryogenic calli were effectively initiated with high concentration of KT and 2,4-D in medium (0.1 mg· L-12,4-D+0.2 mg. L-1 KT or 0.2 mg· L-12,4-D+0.5 mg. L-1 KT).The speed of differentiation and embryogenesis of Xinluzao 33 were faster than that of Xinluzao 32. Embryogenic calli of YZ1 were quickly initiated on media with low concentration(even no) hormone combination; with high concentration hormone of KT, the calli of YZ1 mined brown and went to death easily. The successful regeneration protocol established in this study can be used to improve cotton cultivars by genetic engineering.关键词
棉花/体细胞胚胎/植株再生Key words
Gossypium hirstum L. /somatic embryogenesis /regeneration分类
农业科技引用本文复制引用
刘丽,王娟,王旭文,周小凤,孔宪辉,余渝..新陆早32号、33号的体细胞胚胎发生和植株再生比较研究[J].棉花学报,2011,23(3):259-264,6.基金项目
农业部转基因专项(2008ZX08005-002) （2008ZX08005-002）
