棉花学报2011，Vol.23Issue(3)：279-283,5.
国家棉花区试新品种的SSR指纹图谱分析
SSR Marker Fingerprinting of Cotton Varieties in National Regional Trails
摘要
Abstract
In this study, 57 cotton cultivars in 2009 national regional trails were used as plant materials for DNA fingerprinting.Among 1800 pairs of primers, 22 pairs with steady polymorphism were selected. Totally, 145 bands were observed, and 106 (72.7％) were polymorphic, with an average of 4.8 bands per primer. The mean PIC value of these markers was 0.661, and a DNA fingerprint database for the varieties tested was developed. UPGMA cluster analysis of Genetic similarity showed that the observed 106 polymorphic markers are sufficient to separate all of the 57 varieties from one another at the maximum genetic similar coefficient of 0.98. Cultivars derived from the same district or bred by same institutes were clustered together in different extent, indicating their close genetic relationship.关键词
棉花/区域试验/SSR/DNA指纹图谱Key words
cotton/ national regional trails/ SSR/ DNA fingerprinting分类
农业科技引用本文复制引用
孙宁,杨付新,付小琼,王秀玲,刘逢举,李亚丽,汤磊鹏..国家棉花区试新品种的SSR指纹图谱分析[J].棉花学报,2011,23(3):279-283,5.基金项目
中央级公益性科研院所基本科研业务专项(SJB0901) （SJB0901）
