郑州大学学报（医学版）2011，Vol.46Issue(3)：346-348,3.
顺铂对TRAIL诱导EC1细胞凋亡的增敏效应
Sensitive enhancement of cisplatin on TRAIL-mediated apoptosis in ECl cells
摘要
Abstract
Aim: To investigate the sensitive enhancement of cisplatin( cDDP) on tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-mediated apoptosis in esophageal cancer EC1 cells. MethodS:The effects of TRAIL (10,50, 100,500,and 1 000 ng/L) or cDDP( 1.0,2. 0,4. 0,10. 0,and 20. 0 mg/L) on the cell proliferation of Ecl for 24,48 and 72 h were observed by MTT. EC1 cells were allocated into 4 groups and treated with 100 mg/L TRAIL or 100 mg/L cDDP or combined use of the 2 drugs for 48 h, and the cells not being treated were control. The apoptosis and cell cycles of EC1 were measured by flow cytometry. ResultS;TRAIL did not apparently influence the proliferation of EC1 cells( P > 0. 05) , however, CDDP inhibited the proliferation of EC1 cells(P <0.05) after 24,48 and 72 h treatment. TRAIL had no influence on the cell apoptosis rate of EC1 ( FTRAlL. =5. 836, P =0. 250) , while cDDP could increase the cell apoptosis rate (FcDDP =20. 763,P =0.041). Furthermore, the cell apoptosis rate was greatly increased when the 2 drugs were combined (Fbath, = 57.422,P<0.001). TRAIL or cDDP single had no effects on cell cycle, while combined use of the 2 drugs decreased the percentages of Go/G1 and S phase cells and increased the percentage of G2/M phase cells( P < 0. 05). Conclusion ;cDDP has sensitive enhancement on TRAIL-mediated apoptosis in EC1 cells. TRAIL in combination with cDDP induces EC1 apoptosis and cell cycle to arrest at G2/M phase.关键词
肿瘤坏死因子相关凋亡诱导配体/顺铂/EC1细胞/细胞凋亡/细胞周期Key words
tumor necrosis factor-related apoptosis-inducing ligand/cDDP/Ecl /cell apoptosis/ cell cycle分类
医药卫生引用本文复制引用
江亚南,马俊芬,黄幼田,李沛,李芳芳,杨浩,董子明..顺铂对TRAIL诱导EC1细胞凋亡的增敏效应[J].郑州大学学报（医学版）,2011,46(3):346-348,3.基金项目
郑州大学学生创新实验基金资助项目 2009qnz007 （）
