郑州大学学报（医学版）2011，Vol.46Issue(3)：364-366,3.
RNA干扰对MCF-7细胞eIF4E表达的影响
Effects of RNAi on expression of eukaryotic initiation factor 4E in MCF-7 cells
摘要
Abstract
Aim:To investigate the effect of RNA interfering( RNAi) on the expression of eukaryotic initiation factor 4E(Eif4e) gene in human breast cancer MCF-7 cells. Methods;To design the oligonucleotide contraposed to Eif4e gene, siRNA of Eif4e gene was synthesized by in vitro transcription. Three different concentrations of Eif4e siRNA( 100,150, 200 mg/L) were used to transfect MCF-7 cells in different time periods(24,48,72 h) , and the normal control group, empty liposome group, non-specific siRNA group were established accordingly. The expressions of Eif4e protein and Mrna of each group were detected by using immunocytochemistry and in situ hybridization. Results: After transfecting, in MCF-7 cells,there was a significant decrease in the Eif4e protein and Mrna level in every specific siRNA transfection group of different times, and they were positively correlated with the concentrations of siRNA and the exposure time(P <0.01) ,especially the group of 72 h and the concentration of 200 mg/L(P <0. 05). Conclusion;RNAi can effectively inhibit the expression of Eif4e gene in MCF-7 cells.关键词
真核起始因子4E/乳癌/MCF-7细胞/RNA干扰Key words
eukaryotic initiation factor 4E/breast cancer/ MCF-7 cell/RNA interfering分类
医药卫生引用本文复制引用
崔莹,孙淼淼,崔黎,张威,周强,陈奎生..RNA干扰对MCF-7细胞eIF4E表达的影响[J].郑州大学学报（医学版）,2011,46(3):364-366,3.基金项目
河南省基础与前沿技术研究计划基金资助项目 092300410022 （）
