郑州大学学报（医学版）2011，Vol.46Issue(3)：439-442,4.
DPC4基因转染对胰腺癌JF305细胞凋亡的影响
Effects of DPC4 gene on apoptosis of JF305 cells
邵丽春 1郭晓钟 2徐建华 2邓国伟3
作者信息
- 1. 解放军第463医院消化内科,沈阳,110042
- 2. 沈阳军区总医院消化内科,沈阳,110016
- 3. 沈阳军区空军门诊部内科,沈阳,110015
- 折叠
摘要
Abstract
Aim: To explore the effect of DPC4 gene on the cell apoptosis of human pancreatic carcinom JF305 cells. Methods;Human pancreatic adenocarcinoma cell line JF3O5 was transfected with pBK-CMV-DPC4 plasmid using lipo-fectamine transfection technique, and cells transfected with pBK-CMV plasmid or without transfection were the controls. The expression of DPC4 protein were studied by Western blot. The cell growth was estimated by MTT method. The expressions of Bcl-2 and Bax proteins were detected by flow cytometry. Results: Compared with those of the controls, the proliferation of the cells transfected with pBK-CMV-DPC4 was suppressed significantly, and maintained in Gl phase(F=41 722.447, P < 0. 001) , the expression of DPC4 protein was higher ( F = 10 821. 545 , P < 0. 001) , the Bcl-2 expression was lower ( F = 387. 173,P<0. 001) ,the Bax expression was higher( F = 250. 808 ,P <0. 001) ,and Bcl-2/Bax was lower( F = 776. 568 , P < 0.001). Conclusion: DPC4 could induce JF305 cell apoptosis possibly through regulating the expression of Bcl-2 and Bax proteins.关键词
胰腺癌/DPC4/凋亡/JF305细胞Key words
pancreatic carcinoma/ DPC4 apoptosis/ JF3OS cell分类
医药卫生引用本文复制引用
邵丽春,郭晓钟,徐建华,邓国伟..DPC4基因转染对胰腺癌JF305细胞凋亡的影响[J].郑州大学学报（医学版）,2011,46(3):439-442,4.
