南京林业大学学报（自然科学版）2011，Vol.35Issue(3)：106-110,5.DOI:10.3969/j.issn.1000-2006.2011.03.022
木聚糖酶分级对高温降解木聚糖酶水解的影响
Enzymatic hydrolysis of high-temperature degraded xylan with xylanase
摘要
Abstract
In order to increase xylo-oligosaccharides in the enzymatic hydrolysate with a degree of polymerization (DP)between 2 and 5, crude xylanase was ultrafiltrated to hydrolyze high-temperature degraded xylan. The results showed that three xylanase components, named xylanase A (MW 30-50 ku), xylanase B (MW 10-30 ku) and xylanase C (MW ＜ 10 ku), were obtained after ultrafiltration fraction of crude xylanase. These xylanase components were able to hydrolyze the high-temperature degraded xylan efficiently to xylo-oligosaccharides with a DP between 2 and 5. Xylanase B performed much better than xylanase A and C; 1 mg protein of xylanase B could produce xylobiose 2. 63 g, xylotriose 1.85 g, xylotetraose 1.15 g and xylopentaose 0. 46 g关键词
木聚糖酶/低聚木糖/木聚糖/超滤/水解Key words
xylanase/ xylo-oligosaccharide/ xylan/ ultrafiltration/ hydrolysis分类
化学化工引用本文复制引用
刘蕾,勇强,余世袁..木聚糖酶分级对高温降解木聚糖酶水解的影响[J].南京林业大学学报（自然科学版）,2011,35(3):106-110,5.基金项目
江苏省科技支撑计划(BE2010732) （BE2010732）
国家林业局林业公益性行业科研专项(200904017) （200904017）
江苏省高校科技创新团队资助项目 （）
