农业生物技术学报2011，Vol.19Issue(2)：270-277,8.DOI:10.3969/j.issn.1674-7968.2011.02.011
中国小麦地方品种籽粒强休眠特性的主效基因鉴定
Investigation of Main Loci Contributing to Strong Seed Dormancy of Chinese Wheat Landrace
摘要
Abstract
Many Chinese wheat landraces appear typically strong seed dormancy and pre-harvest sprouting (PHS) tolerance, which are presumed to be controlled by one or two main genes. In this study, we tried to investigate the genetic basis for strong seed dormancy of wheat (Triticum aestivum) landrace, Wanxianbaimaizi, using two recombinant inbred line (RIL) populations from the crosses of Wanxianbaimaizi/Jing 411 and Wanxianbaimaizi/ Zhongyou 9507, respectively. The populations were genotyped with 1 754 SSR markers and a gene-marker (Vp1-b2). By analyzing the data of grain dormancy covering two sites and four years, two major QTLs were detected using composite interval mapping, one was on chromosome 3AS flanked by Xbarc57 and Xbarc294 with linkage distances of 5.8 cM and 8.5 cM in the two populations, and the other on 3BL linked to Vp1 and Xwmc446 with the distance of 8.1 cM. The two QTLs were designated Qsd. ahau-3A and Qsd. ahau-3B, which explained 25.6％ ～ 48.3％ and 23.5％ ～ 37.8％ of phenotypic variation in the two mapping populations across four environments, respectively. These results demonstrated that the long period seed dormancy of Wanxianbaimaizi is due to the two loci with good stability in varied environments; Therefore, it is possible to obtain strong seed dormancy and resistance to PHS in white wheat cultivars by pyramiding the two major genes.关键词
小麦/籽粒休眠/中国地方小麦品种/RKILs群体/QTL定位Key words
Common wheat/ Seed dormancy/ Chinese wheat landraee/ RILs/ QTL引用本文复制引用
张海萍,冯继明,常成,马传喜,张秀英,闫长生,游光霞,肖世和..中国小麦地方品种籽粒强休眠特性的主效基因鉴定[J].农业生物技术学报,2011,19(2):270-277,8.基金项目
This work was supported by Grants Scientific Research Special Program of Agricultural Commonweal (No.nyhyzx07-002),Natural Science Research of Anhui Province (No.KJ2008B21),Excellent Youth with Ability of University of Anhui Province (No.2009SQRZ035ZD) and Youth Science fund of Anhui Agricultural University (No.2009zr22) （No.nyhyzx07-002）
