农业生物技术学报2011，Vol.19Issue(2)：287-293,7.DOI:10.3969/j.issn.1674-7968.2011.02.013
高温诱导体外培养奶牛乳腺上皮细胞的应激响应
Responses of Cultmed Bovine Mammary Epithelial Cells to Heat Stress
摘要
Abstract
The experiment was conducted to study the response of bovine mammary epithelial cells induced by heat stress.Bovine mammary epithelial cells were exposed to 42 ℃ as the heat stress model and 38℃ as consol, the transcription of targeted genes including heat shock proteins (HSPs) family, apoptosis, mlik protein and fatty acid biosynthesis, were detected by RT-qPCR.Results showed that the mRNA expression level of hsp27, hsp70 and hsp90, and heat shock factor-1 (hsf-1) increased after 0.5 h at 42 ℃, and among them hsp70 increased sharply, then decreased with the extended time oftreatment.The apoptosis genes Bcl-2 and Bax had different transcription, Bcl-2 upregulated during 24 h, while Bax upregulated first and downregulated later.Heat stress decreased the mRNA level of milk protein and fatty acids genes (α- and β-casein(CSN2), butyrophilin(BTN), and fatty acid synthase), increased transcription of fatty acid biosynthesis correlative genes (acetyl CoA carboxylase (A CA CA), proxiomepro liferater activatedreceptor-gama (PPARG), and sterol response element binding protein (SREBF-1)), but did not influence the expression of roxiomepro liferater activatedreceptor-alfa(PPARA).It is suggested that heat shock induced the stress responses of bovine mammary peithelial cells, remarkably impacted the secretion of milk protein and fat, and arose tolerance of cell itself.关键词
奶牛乳腺上皮细胞/热应激/热休克蛋白/酪蛋白/乙酰酶A羧化酶/嗜乳脂蛋白/脂肪酸Key words
Bovine mammary epithelial cells/ Heat shock/ Heat shock proteins/ Casein/ Acetyl-coenzyme A carboxylase alpha/ Butyrophilin/ Fatty acids引用本文复制引用
胡菡,王加启,李发弟,卜登攀,周凌云,崔瑞莲..高温诱导体外培养奶牛乳腺上皮细胞的应激响应[J].农业生物技术学报,2011,19(2):287-293,7.基金项目
本研究由国家"十一五"科技支撑计划项目(No.2006BAD04A14)资助 （No.2006BAD04A14）
