农业生物技术学报2011，Vol.19Issue(2)：323-329,7.DOI:10.3969/j.issn.1674-7968.2011.02.018
牦牛瘦素受体基因(LEPR)多态性分析
Polymorphism of Leptin Receptor Gene (LEPR) in Yak (Bos grunniens)
摘要
Abstract
Leptin receptor (LEPR) plays a pivotal role in regulating leptin function as leptin binding protein.Variation in the LERP gene has been associated with obesity in human and fat deposition in cattle, swine and other animals. In this study, three pairs of primers were designed respectively referring to bovine(Bos tauras) LERP exon 4, exon 5 and exon 8 as well as their contiguous parts sequence in GenBank, so as to investigate the variations in corresponding part of LERP gene in Tianzhu white yak, Gannan yak and Qinghai yak (Bos grunniens) by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis. The polymorphisms were observed at LERP exon 4 and exon 8 as well as their contiguous parts in yak. Two allelic variations containing five single nucleotide polymorphisms(SNPs) were observed including two missense mutations at LERP exon 4 and contiguous parts in three yak populations. Allele A was the most common allele and the polymorphism was low with PIC less than 0.25 at this region of LEPR gene in yak. Five SNPs including three missenses mutations were checked at LERP exon 8 and contiguous parts in yak, but alleles distributed disproportionably among three yak populations, such as alleles A→F observed in Tianzhu white yak. but alleles A→C in Gennan yak and Qinghai yak Allele A was the most common allele, and that allelic and genotype frequency were significantly deviated from Hardy-Weinberg balanced (P＜0.05) The polymorphism was higher or moderate with PIC more than 025 at LERPexon 8 in three yak populations No polymorphism was found at LERPexon 5 and contiguous parts,but three SNPs were observed at intron 4 and intron 5 relative to bovine LERP gene sequence. Exon 8 for LEPR gene codes the amino acids consisting of C2 domain of LEPR where amino acids recognizes and binds ligand, for LEPR gene with much polymorphism, exon 8 would become a potential locus for genetic markers, in which it may be possible for traits regulation.关键词
牦牛/瘦素受体基因(LEPR)/PCR-SSCP/SNPsKey words
Yak/ Leptin receptor gene(LEPR)/PCR-SSCP/ SNPs引用本文复制引用
闫伟,罗玉柱,杨勤,成述儒,胡江..牦牛瘦素受体基因(LEPR)多态性分析[J].农业生物技术学报,2011,19(2):323-329,7.基金项目
本研究由国家科技支撑计划课题(No.2007BAD52805和No.2009BAC53806)、甘肃省重点实验室建设计划项目(No.085RTSA004)和甘肃省发改委项目共同资助 （No.2007BAD52805和No.2009BAC53806）
