农业生物技术学报2011，Vol.19Issue(2)：335-341,7.DOI:10.3969/j.issn.1674-7968.2011.02.020
重组脂联素(rAdp)对皖南花猪骨骼肌卫星细胞脂联素及受体和肌球蛋白重链基因表达的影响
Effect of Recombinant Adiponectin (rAdp) on Adiponectin, Adiponectin Receptors and Myosin Heavy Chain (MyHC) mRNA Expression in Muscle Satellite Cell of Wannanhua Pigs In vitro
摘要
Abstract
Adiponectin (Adp) is a cytokine secreted mainly by adipose tissue. The funcfon of Adp has attracted recent interest. Skeletal muscle satellite cells were separated from semitendinosus muscle of Wannanhua pig at 10 days of age. Muscle satellite cells were treated with 0, 1, 5 and 10 μg/mL recombinant adiponectin (rAdp)respectively for 12 h or 24 h. Adp, AdpR1, AdpR2, four isoforms of myosin heavy chain (MyHCI, MyHC2a,MyHC2b and MyHC2x), AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor alpha (PPARa) mRNA levels were determined by Real-time fluorescence quantitative RT-PCR. The results showed that: (1) mRNA expression of Adp and AdpR1 treated with 1 μg/mL rAdp was significantly increased,and AdpR2 mRNA expression was even significantly increased only at 24 h. But mRNA expression of Adp,AdpR1 andAdpR2 treated with 5 and 10 μg/mL rAdp were lower; (2) mRNA expression of MyHC1 and MyHC2x treated with 1 μg/mL rAdp were significantly increased, but MyHC2b mRNA expression was significantly decresed only at 24 h. mRNA expression of MyHC2b treated with 5 and 10 μg/mL rAdp was significantly decresed, but MyHC1 mRNA expression had no significant change, mRNA expression of MyHC2a treated with 10 μg/mL rAdp was significantly decresed. MyHC2x mRNA expression treated with 5 μg/mL rAdp was incrcsed significantly. (3) mRNA expression of AMPK treated with 1, 5 and 10 μg/mL rAdp was significantly increased only at 12 h. However, the low rAdp doseage group had higher AMPK mRNA level. PPARa mRNA expression was significantly increased treated with 1 μg/mL rAdp. But high rAdp dosage groups had no this effects. The results suggest that the function of adiponectin to skeletal muscle may be related to activating both AMPK and PPARa signal pathways by binding adiponectin receptor. Adiponectin can change composition of muscle fibers by increasing MyHC1, MyHC2a and MyHC2x, and decreasing MyHC2b gene expression.关键词
脂联素/猪骨骼肌卫星细胞/脂联素受体/肌球蛋白重链(MyHC)Key words
Adiponectin/ Porcine skeletal muscle satellite cell/ Adiponectin receptor/ Myosin heavy chain (MyHC)引用本文复制引用
张佳,周杰,盛晟,邵康,吴小雪,李维新,殷宗俊..重组脂联素(rAdp)对皖南花猪骨骼肌卫星细胞脂联素及受体和肌球蛋白重链基因表达的影响[J].农业生物技术学报,2011,19(2):335-341,7.基金项目
本研究由国家自然科学基金项目(No.30771581)和安徽省十一五科技攻关计划重大科技专项(No.08010301079)共同资助 （No.30771581）
