畜牧与兽医2011，Vol.43Issue(6)：1-4,4.
表达NDV HN基因的重组鸡痘病毒的部分生物学特性研究
Biological characteristics of a recombinant fowlpox virus expressing Newcastle disease virus HN gene
摘要
Abstract
To evaluate the effect of transferring gene on biological characteristics of fowlpox virus ( FPV) , a recombinant fowlpox virus expressing newcastle disease virus ( NDV) HN gene ( rFPV-12LSHN) was subjected to ultrastructural analysis. The result demonstrated that the morphology of mature virions. replication pattern. and production of rFPV-12LSHN in infected cells were similar to those of FPV. When ll-day-old chicken embryonated eggs were inoculated with rFPV by chonoallantoic membrane ( CAM) route, typical pock lesions were found on the CAM. and the chicken embryo minimal infecting dose was less than 100 PFU. The expression of NDV HN gene in rFPV could be stably detected by immunofluorescence assay when rFPV was passaged in chicken embryo fibroblast ( CEF) cells. The SPF chickens inoculated with 103 PFU of rFPV-12LSHN were completely protected from the virulent NDV strain at 3 weeks after vaccination.关键词
新城疫病毒/重组鸡痘病毒/形态学/最小感染量/HN基因Key words
Newcastle disease virus/ recombinant fowlpox virus/ morphology/ minimal infecting dose/ HN gene分类
农业科技引用本文复制引用
甘军纪,刘武杰,彭大新,吴艳涛,刘秀梵..表达NDV HN基因的重组鸡痘病毒的部分生物学特性研究[J].畜牧与兽医,2011,43(6):1-4,4.基金项目
国家863计划(2006AA10A205) （2006AA10A205）
江苏省高技术研究计划(BG2007336). （BG2007336）
