物理化学学报2011，Vol.27Issue(6)：1525-1530,6.
微量热法研究环境NaCl浓度对盐生盐杆菌生长代谢的影响
Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobium
摘要
Abstract
Microcalorimetry was used to study the influence of extracellular NaCl concentration on the growth metabolism of Halobacterium halobium (Hbt. halobium) using a TAM air isothermal microcalorimeter. The metabolic thermogenic curves of Hbt. halobium growth in different concentrations of NaCl were obtained using the ampoule method. The thermokinetic equations and parameters of Hbt.halobium growth were calculated and the relationship between each thermokinetic parameter and the concentration of NaCl was obtained. The results showed that the optimum NaCl concentration for Hbt.halobium growth was not a wide range from 3.5 mol L-1 to about 5.2 mol · L-1 (NaCl saturation), as is generally acknowledged, but just around 3.9 mol· L-1. For an extracellular NaCl concentration above 3.9 mol · L-1, the growth metabolism of Hbt. halobium decreased constantly with an increase in the NaCl concentration. Further investigation by transmission electron microscopy revealed that the Hbt. halobium cells growing in approaching NaCl saturation underwent plasmolysis, which interpreted the finding of microcalorimetry perfectly. All these results led to a new interpretation of the structural transformations of Hbt. halobium upon NaCl concentration altering.关键词
微量热/嗜盐古生菌/环境NaCl浓度/细胞表面结构Key words
Microcalorimetry/ Haloarchaea/ Extracellular NaCl concentration/ Cell surface structure分类
化学化工引用本文复制引用
曾驰,朱建裕..微量热法研究环境NaCl浓度对盐生盐杆菌生长代谢的影响[J].物理化学学报,2011,27(6):1525-1530,6.基金项目
国家自然科学基金(31000050,30700008)和湖北省教育厅科学技术研究项目(B20111701)资助 （31000050,30700008）
