中国药理学通报2011，Vol.27Issue(9)：1259-1262,4.DOI:10.3969/j.issn.1001-1978.2011.09.018
卡托普利对MC3T3-E1细胞增殖、分化及Ⅰ型胶原mRNA表达的影响
Effects of captopril on the proliferation,differentiation and the mRNA expression of Type Ⅰ Collagen in MC3T3-E1 cells
摘要
Abstract
Aim To investigate the effects of captopril on the proliferation. differentiation and the mRNA expression of Type Ⅰ Collagen( COLL- Ⅰ ) in MC3T3-E1 cells. Methods The MTT method was employed to detect the cells proliferation after culture with captopril for 12 h.24 h and 36 h respectively. The cell differentiation was evaluated by detecting activities of alkaline phosphatase( ALP ) using PNPP method after culture with captopril for 3 d,5 d and 7 d respectively. Reverse transcription-Polymerase chain reaction ( RTPCR ) was used to screen the level of Type Ⅰ Collagen mRNA expression after incubation with captopril for 7 d. Results Captopril accelerated cell growth with 10 -11 mol · L-1 concentration after 24 h. After 7 days of stimulation with 10 -11 mol · L-1 captopril, ALP activity increased significantly. Captopril up-regulated COLL- Ⅰ mRNA expression at a concentration of l0-11 mol · L-1. Conclusion Captopril stimulates the differentiation, proliferation and up-regulates COLL- Ⅰ mRNA expression of MC3T3-E1 cells in vitro.关键词
卡托普利/MC3T3-E1细胞/增殖/分化/Ⅰ型胶原/骨质疏松Key words
captopril/ MC3T3-E1 cells/ differentiation / proliferation / Type Ⅰ C ollagen / osteoporosis分类
医药卫生引用本文复制引用
刘钰瑜,姚卫民,徐道华,崔燎..卡托普利对MC3T3-E1细胞增殖、分化及Ⅰ型胶原mRNA表达的影响[J].中国药理学通报,2011,27(9):1259-1262,4.基金项目
广东省自然科学基金资助项目(No 10152402301000000) （No 10152402301000000）
广东省教育厅育苗工程项目(No 08-NYM-061) （No 08-NYM-061）
