中国药理学通报2011，Vol.27Issue(9)：1267-1271,5.DOI:10.3969/j.issn.1001-1978.2011.09.020
蛇床子素对脑缺血/再灌注大鼠海马LTP及氨基酸含量的影响
Effects of osthole on LTP and contents of amino acid in hippocampus of cerebral ischemia-reperfusion rats
摘要
Abstract
Aim To observe the effects of osthole on synaptic transmission activities and contents of amino acid in hippocampus of cerebral ischemia-reperfusion rats. Methods SD rats were randomly divided into normal group , sham-operated group , model group , osthole groups ( 25. 0, 12. 5 mg · kg-1 ) and nimodipine group( 1. 0 mg · kg-1 ). Rat model of middle cerebral artery occlusion ( MCAO ) was copied and given reperfusion after 2 h. The infarct volume was shown with 2,3,5-triphenyl tetrazolium chloride( TTC ) staining. The spatial learning and memory ability of rats was measured by Morris water maze. Electrophysiological recordings were conducted to detect the effects of osthole on phenomena of synaptic transmission long-term potentiation ( LTP )in dentate gyrus ( DG ) of hippocampus. The contents of amino acids in hippocampus at different time after reperfusion were detected by high performance liquid chromatography ( HPLC ). Results The infarct volume in osthole group ( 25. 0, 12. 5 mg ·kg-1 ) was lower than that in model group( P ＜ 0. 05 ).Osthole dose-dependently shortened the time of seeking platform and significantly improved the LTP induced by high frequency stimulation( HFS ) in the DG of hippocampus. The content of Glu in osthole groups( 25.0,12. 5 mg · kg-1 ) was significantly lower than that in model group( P ＜ 0. 05 ). Compared with model group ,the content of GABA was decreased in osthole groups ( 25. 0. 12. 5 mg · kg-1 ) only at th after reperfusion,while at 72 h after reperfusion osthole ( 25.0 mg ·kg-1 ) increased the content of CABA ( P ＜ 0. 05 ).This result of first decrease and then increase also appeared in nimodipine group. Conclusion Osthole can improve learning and memory impairment and enhance hippocampal LTP in cerebral ischemia-reperfusion rats. which is related to the regulation to Glu and GABA levels.关键词
蛇床子素/脑缺血/再灌注/学习记忆/长时程增强/谷氨酸/γ-氨基丁酸Key words
osthole/ cerebral ischemia-reperfusion/learning and memory/ long-term potentiation/ glutamic acid/ γ-aminobutyric acid分类
医药卫生引用本文复制引用
董晓华,张丹参,张力,李炜..蛇床子素对脑缺血/再灌注大鼠海马LTP及氨基酸含量的影响[J].中国药理学通报,2011,27(9):1267-1271,5.基金项目
河北省卫生厅资助课题(No 20090183) （No 20090183）
河北省自然科学基金资助项目(No 2004000653) （No 2004000653）
