中国药理学通报2011，Vol.27Issue(9)：1317-1321,5.DOI:10.3969/j.issn.1001-1978.2011.09.032
经前舒颗粒对郁怒反应模型大鼠海马脑区基因表达谱的影响
Effect of Jingqianshu granule on gene expression profile in the hippocampal of the anger-in rat model
摘要
Abstract
Aim To elucidate the medicine targets and molecular mechanism of Jingqianshu granule, one Chinese herbal formulae. on the hippocampal of anger-in rat model and to identify the gene expression profile by using gene-chip technique. Methods The anger-in animal models were prepared using a social isolation plus resident-intruder stress. Affymetrix rat genome 230 2. 0 chips were used to analyze gene expression profiles of hippocampus brain in control rat group , anger-in emotion rat model group and Jingqianshu granule treatment group. The results were analyzed by bioinformatics methods. Differential expression gene Htr3b and CABABR2 were validated by the RT-PCR test. Results Compared with normal group, 127 genes closely related with anger-in were screened by detecting hippocampus of other groups. Jingqianshu granule treatment group versus anger-in rat model, 55 and 72 genes in anger-in emotion rat model were up-regulated and down-regulated in Jingqianshu granule treatment group respectively. mainly associated with receptors of various hormones, signal transducins. metabolic enzyme,various ion channel protein, transport protein, cytokines receptor etc. The genes involved neuroactive ligand-receptor interaction and MAPK signaling regulated pathways, etc. The results of RT-PCR test of Htr3b and GABABR2 gene expression were in accord with gene chips data. Conclusions The emotion responses of anger-in can lead to changes of gene expression of Htr3b, Fgfr2, Grm8, Gmeb2 etc in the hippocampus.To some extent, Jingqianshu granule can improve the abnormal ethology of model rat by up-regulating and down-regulating these genes or regulated neuroactive ligand-receptor interaction etc pathways in hippocampus brain of the treatment group versus anger-in rat model. These results provide direction and clues for further research.关键词
经前舒颗粒/郁怒/大鼠/基因表达谱/海马/分子作用机制Key words
Jingqianshu granule / anger-in / rats / gene expression profile/ hippocampus/ molecular action mechanism分类
医药卫生引用本文复制引用
郭英慧,高杰,徐凯勇,宋春红,乔明琦..经前舒颗粒对郁怒反应模型大鼠海马脑区基因表达谱的影响[J].中国药理学通报,2011,27(9):1317-1321,5.基金项目
国家重点基础研究发展计划(973计划)资助项目(No 2011CB505102) （973计划）
国家自然科学基金重点项目(No 30930110) （No 30930110）
国家自然科学基金面上项目(No 30973688) （No 30973688）
中国博士后科学基金面上资助项目(No 20090451343) （No 20090451343）
山东省博士后创新项目专项基金资助项目(No 200803033) （No 200803033）
