中国动脉硬化杂志2011，Vol.19Issue(6)：474-478,5.
钩藤总生物碱对D-半乳糖诱导的内皮细胞衰老的干预
Uncaria Alkaloids' Intervention on the Aged Endothelial Cell Induced by D-Galactose
摘要
Abstract
Aim To investigate the effect of Uncaria alkaloids on protecting rat vascular endothelial cells and inhibiting cell senescence. Methods Aging model of rat aortic endothelial cells (REAC) was established by D-galactose. Treated with Uncaria alkaloids and Captopril, separately, then, the aging vascular endothelial cell morphology were observated by scanning electron microscopy, vascular endothelial cell senescence rate was determinated by β-galactosidase staining method, and telomerase activity was determinated by PCR-ELISA method. Results REAC cell morphology treated with Uncaria alkaloids or captopril was significantly improved, β-galactosidase expression and telomerase activity were significantly decreased (P ＜ 0.05). The effects of each dose group of Uncaria alkaloids were: medium dose ＞ low dose ＞ high dose, suggesting that high dose of Uncaria alkaloids ( ＞ 400 mg/L) may have a certain degree of cytotoxicity,and the best dose of Uncaria alkaloids dose was 200 mg/L. Conclusion Uncaria alkaloids can inhibit rat aortic endothelial cell senescence, and protect vascular endothelium.关键词
血管内皮细胞/细胞衰老/D-半乳糖/β-半乳糖苷酶/端粒酶Key words
Rat Aortic Endothelial Cells/ Cell Senescence/ D-Galactose/ β-Galactosidase/ Telomerase分类
医药卫生引用本文复制引用
姜月华,李运伦,赵婧,霍青..钩藤总生物碱对D-半乳糖诱导的内皮细胞衰老的干预[J].中国动脉硬化杂志,2011,19(6):474-478,5.基金项目
山东省教育厅资助课题(J05L05)、山东省泰山学者建设工程专项资助 （J05L05）
