中国动脉硬化杂志2011，Vol.19Issue(6)：493-498,6.
过表达Syndecan-4促进人脐静脉内皮细胞增殖
Syndecan-4 Overexpression Enhances Human Umbilical Vein Endothelial Cells Proliferation
宗斌 1谢峻 1徐标1
作者信息
- 1. 南京大学医学院附属鼓楼医院心脏科,江苏省南京市,210008
- 折叠
摘要
Abstract
Aim The proliferation of endothelial cells plays an important role in angiogenesis. In present study,we observed the effect of syndecan-4 on human umbilical vein endothelial cells (HUVECs) proliferation as well as its possible mechanism. Methods HUVECs were isolated and cultured. The cells were infected with Ad-syndecan-4 or Adnull as control. Immunostaining was performed to investigate syndecan-4 expression and clusterization. Western blotting was used to assess phosphorylation of downstream kinase Akt and endothelial nitric oxide synthase (eNOS). Proliferation ability of HUVECs was assessed by p-H3, bromodeoxyuridine (5-BrdU) and 5-ethynyl-2'-deoxyuridine (EdU). Results Compared with the Ad-null group, increased cell proliferation rate was observed in the Ad-syndecan-4 group. Syndecan-4 overexpression induced the clusterization of syndecan-4 themselves. The levels of Akt and eNOS phosphorylation were also increased in HUVECs with syndecan-4 overexpression. Conclusion Overexpressed syndecan-4 induced the self-clusterization of syndecan-4 and the activation of downstream Akt and eNOS, which may contribute to increased proliferation of HUVECs.关键词
血管新生/Syndecan-4/人脐静脉内皮细胞/增殖Key words
Angiogenesis/ Syndecan-4/ Human Umbilical Vein Endothelial Cell/ Proliferation分类
医药卫生引用本文复制引用
宗斌,谢峻,徐标..过表达Syndecan-4促进人脐静脉内皮细胞增殖[J].中国动脉硬化杂志,2011,19(6):493-498,6.
