高等学校化学学报2011，Vol.32Issue(6)：1277-1283,7.
大黄酸铜(Ⅱ)配合物与牛血清白蛋白的相互作用
Interaction of Rhein-Cu(Ⅱ) Complexes with Bovine Serum Albumin
摘要
Abstract
The interaction between Rhein-Cu and bovine serum albumin (BSA) was investigated by fluores cence, ultraviolet and circular dichroism spectra in conjunction with electrochemical methods. The results in dicate that Rhein-Cu leads to the intrinsic fluorescence quenching of BSA through a static quenching proce dure. The binding constants K and binding site n at 298 and 309 K were obtained. The binding locality was found to be an area of 3.21 nm away from tryptophan residue in BSA based on FOrster non-radiation energy transfer mechanism. The experimental results show that Rhein-Cu can be inserted into the BSA, quenching the inner fluorescence by forming Rhein-Cu/BSA complex. The binding power between Rhein-Cu and BSA is mainly hydrophobic interaction according to the thermodynamic parameters and the electrochemical behaviors of Rhein-Cu. The effect of Rhein-Cu on the conformation of BSA was also analyzed.关键词
大黄酸铜/牛血清白蛋白/荧光光谱/紫外光谱/圆二色光谱Key words
Rhein-Cu/ Bovine serum albumin(BSA)/ Fluorescence spectrum/ Ultraviolet spectrum/ Circular dichroism spectrum (Ed.: I, K)分类
化学化工引用本文复制引用
赵芳,梁慧,程惠,王军,赵文慧..大黄酸铜(Ⅱ)配合物与牛血清白蛋白的相互作用[J].高等学校化学学报,2011,32(6):1277-1283,7.基金项目
国家自然科学基金(批准号:20961009)资助. （批准号:20961009）
