中国海洋大学学报（自然科学版）2011，Vol.41Issue(6)：61-67,7.
黄海冷水团可培养细菌的多样性研究
Diversity of Cultivable Bacteria in the Yellow Sea Cold Water Mass
摘要
Abstract
Phylogenetic diversity of cultivable bacteria from the inter of the Yellow Sea Cold Water Mass (YSCWM) area was studied in July 2008, and was compared with that from the exterior of YSCWM area.Sea water samples were collected in five stations 3500-5, 3600-6, 3600-8, 3400-6 and 3800-2 from the inter of YSCWM, and two stations 3200-3 and 3850-5 from the exterior of YSCWM. According to the AODC and DVC results, the total bacterial number and the total number of viable cells of the marine water samples were (1. 3～4. 8) × 105/mL and (0. 6～1.6) × 105/mL, respectively; from the colony count method with 2216E plates, the abundance of the heterotrophic bacteria of sea water was (0. 56～2. 2) × 103cfu/mL. 475 bacterial strains were isolated from 2216E plates, and the PCR-ARDRA technique was performed on these strains for cluster analysis, with fragments of 16S rDNA digested by restriction enzyme Hha I. The 16S rDNA of all major RFLP patterns were sequenced. Phylogenetic analysis of 114 bacterial strains showed that bacterial phylotypes from the inter of YSCWM were made up of four phyla bacteria, i. e. Proteobacteria, Actinobacteria, Firmicutes and Flavobacteria, including 24 genera, and from the exterior were made up of the same four taxon, including 15 genera. α-,β-and γ-Proteobacteria subdivisions of the Proteobacteria were discovered from the inter of YSCWM. However β-Proteobacteria was not discovered from the exterior of YSCWM. γ-Proteobacteria represented the vast majority of bacterial isolated at every depth. By comparing, α-Proteobacteria represented higher proportion with percentage of 26. 8％ inter of YSCWM and lower proportion with percentage of 15. 6％ exterior of YSCWM.These indicated that samples from the inter as well as the exterior of YSCWM showed high bacterial diversity, but bacterial community composition, as well as the predominant bacterial groups, from the inter of YSCWM was different from the exterior of YSCWM. In addition, according to the 16S rDNA gene sequences analysis, 6 strains might be novel species.关键词
可培养细菌/多样性/16S rDNA/分析/黄海冷水团Key words
cultivable bacteria/ diversity/ 16S rDNA analysis/ YSCWM分类
生物科学引用本文复制引用
王红,刘吉文,张晓华..黄海冷水团可培养细菌的多样性研究[J].中国海洋大学学报（自然科学版）,2011,41(6):61-67,7.基金项目
国家高技术研究发展计划项目(2007AA09Z434)资助 （2007AA09Z434）
