河南农业科学2011,Vol.40Issue(4):127-128,133,3.
红树莓组培快繁技术研究
The Tissue Culture Propagation Technology of Red Raspberry
郭玉霞 1董彦琪 1刘喜存 1周联东 1王文洁 1杜春宜1
作者信息
摘要
Abstract
The shoot tips of red raspberry Hongmei 38 were used as the explants for tissue culture in the MS medium with 6 -BA and IBA to study the propagation technology. The result showed that the best medium for inducing germination was MS+ 1.0 mg/L 6 -BA+0.1 mg/L IBA+30.0 g/L sucrose+4.0 g/L AGAR powder;the best medium for subculture was MS+1.5 mg/L 6 -BA +0.03 mg/L IBA+4.0 g/L AGAR powder+30.0 g/L sucrose; the best rooting medium was 1/2 MS+0.2mg/L IBA +0.2% activated carbon+20.0g/L sugar+4.5 g/L AGAR powder. When the seedlings height reached 5 cm with more 5 roots, they were transplanted in greenhouse (sharp sand: peat soil=l: 1) ,and the survival rate was 92.78%.关键词
树莓/组织培养/快繁技术Key words
Raspberry/ Tissue culture/ Rapid propagation分类
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郭玉霞,董彦琪,刘喜存,周联东,王文洁,杜春宜..红树莓组培快繁技术研究[J].河南农业科学,2011,40(4):127-128,133,3.