生物信息学2011，Vol.9Issue(2)：125-130,6.DOI:10.3969/j.issn.1672-5565.2011.02.007
高粱抗坏血酸过氧化物酶基因的电子克隆及序列分析
In Silico Cloning and Characterization of Sorghum APX Gene
摘要
Abstract
The APX gene from Sorghum was cloned by using EST datebase , in silico cloning etc. . APX gene from Sorghum contained an ORF with the longest length of 753bp , and encoding 250 amino acids. The molecular weight and pI of deduced protein is 27. 1kDa and 5. 135. The phylogenetic analysis result showed that the distance in evolution was shortest between it and cytoplasmic APX ( ACG41151) of maize which is C4 plant, and their relationship is the closest. The Sorghum APX has not signal peptide and is a hydrophilic protein locating in cytoplasm. The 33 - 44 amino acids is its peroxidases active site signature , and the 155 - 165 amino acids is its peroxidases proximal heme - ligand signature. The predicted second and tertiary structure indicates that Sorghum APX contains more random coil and α helix and a small quantity of extended strand.关键词
高粱/APX基因/电子克隆/序列分析Key words
sorghum / APX gene / in silico cloning / sequence analysis分类
生物科学引用本文复制引用
陈国强,孟鹏,刘李黎,陈刚,王萍..高粱抗坏血酸过氧化物酶基因的电子克隆及序列分析[J].生物信息学,2011,9(2):125-130,6.基金项目
淮海工学院引进人才科研启动基金项目(KK04017、KK01073). （KK04017、KK01073）
