作物学报2011，Vol.37Issue(6)：1005-1011,7.DOI:10.3724/SP.J.1006.2011.01005
硅及其吸收基因Lsil调节水稻耐UV-B辐射的作用
Silicon and Its Uptaking Gene Lsi1 in Regulation of Rice UV-B Tolerance
摘要
Abstract
Silicon (Si) has beneficial function in enhancing plant resistance to biotic and abiotic stresses. Rice is a typical Si-accumulating plant. In this research, UV-B tolerant rice accession Lemont, UV-B sensitivity rice Dular and their low silicon rice gene 1 (Lsi1) transgenic lines were used to explore the relationships between silicon and rice UV-B tolerance. It was found that rice cultured in Si-deficiency solution had lower gene transcript levels of phenylalanine ammonia lyase (PAL), photolyase (PL), and lower contents of total phenolics and flavonoids in leaves than that in Si-containing solution. The same tendency was found in the case when the rice accessions were supplementarily exposed to UV-B radiation, although both gene expression level and antioxidants concentrations were increased. Further, Lsi1-suppressed or overexpressed transgenic rice lines of Lemont, and Lsi1-overexpressed transgenic rice line of Dular were also detected in the same treatments. The results showed that gene transcript level of PAL and PL was increased in Lsi1-overexpressed transgenic line, but down-regulated in Lsi1-RNAi line of Lemont as compared with their wild types (WT) under normal light condition. The expression level of the two genes in all entries was enhanced after UV-B radiation treatment, and it was the highest in Lsi1-overexpressed line of Lemont, followed by their WT, and lowest in Lsi1-RNAi line. The same tendency was also found in the content of total phenolics and flavonoids. The similar results were further confirmed in the overexpression of Lsi1 in Dular. The findings suggested that rice UV-B tolerance could be effectively mediated by enhancing/inhibiting expression of Lsi1.关键词
硅/硅基因/水稻/紫外线BKey words
Silicon/ Lsi1 / Rice ( Oryza sativa L.)/ Ultraviolet-B引用本文复制引用
方长旬,王清水,余彦,黄力坤,吴杏春,林文雄..硅及其吸收基因Lsil调节水稻耐UV-B辐射的作用[J].作物学报,2011,37(6):1005-1011,7.基金项目
本研究由国家自然科学基金项目(30971737),教育部高等学校博士点专项科研基金项目(20093515110009)和福建省自然科学基金项目(2009J01055)的资助. （30971737）
