中山大学学报(自然科学版)2011，Vol.50Issue(3)：102-104,111,4.
紫苏DNA导入对烟叶中芦丁含量的影响
Effect of Introducing Perilla frutescens(L.)Britt DNA on Rutin Content in Tobacco Leaves
摘要
Abstract
This paper studied the content of rutin in the progenies of flue-cured tobacco with introduced exogenous DNA of Perillafrutescens ( L. ) Britt. The method of sexual hybridization combined with asexual graft was used to introduce exogenous DNA into tobacco, and the optimum environment of pollination was selected. HPLC was adopted to analyze the content of rutin in tobacco leaves. This method could significantly improve the success ratio of introducing exogenous DNA under the condition of lower temperature (22 ～ 25 ℃ ) and higher relative humidity (70 ％ ～ 80 ％ ). The results of the analysis showed that the content of rutin in the progenies increased by 70. 4 ％ compared with that in female parent tobacco leaves and was 4 ～5 times higher than that in buds of Sophora japonica L. from main production area in China. The progenies of flue-cured tobacco with introduced exogenous DNA could provide new materials for mass-extraction of rutin. This study also explored a new way for utilizing gene resources in medicinal plants.关键词
紫苏/烟叶/芦丁Key words
Perilla frutescens ( L. ) Britt/ tobacco/ rutin分类
农业科技引用本文复制引用
魏克强,杨俊仙,魏治中..紫苏DNA导入对烟叶中芦丁含量的影响[J].中山大学学报(自然科学版),2011,50(3):102-104,111,4.基金项目
教育部高等学校博士学科点专项科研基金资助项目(200801081012) （200801081012）
国家烟草专卖局重点科技资助项目(110199901005) （110199901005）
山西省科技产业化环境建设计划资助项目(2006071033) （2006071033）
