作物学报2011，Vol.37Issue(6)：955-964,10.DOI:10.3724/SP.J.1006.2011.00955
水稻半矮秆基因iga-1的鉴定及精细定位
Identification and Fine Mapping of a Semidwarf Gene iga-1 in Rice
摘要
Abstract
A semidwarf gene iga-1 of rice (Oryza sativa L.) by mutagenesis of outer space treatment from Texianzhan 13 was identified. The dwarf lines CHA-2 and CHA-2N which carried iga-1 showed great variation in agronomic traits. On the basis of the internode length of CHA-2 and CHA-2N, the mutant belongs to the dn type of dwarfing. GA3 treatment, endo-GA3 measurement and α-amylase activity analysis in endosperm showed that iga-1 is independent of gibberellin acid. Using a large F2 population derived from a cross between the CHA-2 and an japonica rice variety, 02428, the iga-1 gene was fine mapped into a 32.01 kb physical distance between two InDel markers, DL18 and DL19 on chromosome 5, where five open reading frames were predicted,one of which was the rice gibberellin-insensitive dwarf mutant gene D1. Sequence analysis showed that no variation in D1 locus was detected among CHA-2, CHA-2N and Texianzhan 13. Thus, D1 can not be the candidate gene of iga-1. Comparing the other dwarf genes on chromosome 5 showed that iga-1 is possibly allelic to the semidwarf gene sd-7.关键词
水稻/半矮秆突变体/iga-1基因/精细定位Key words
Rice/ Semidwarf mutant/ iga-1/ Fine mapping引用本文复制引用
郭涛,霍兴,饶得花,刘永柱,张建国,陈志强,王慧..水稻半矮秆基因iga-1的鉴定及精细定位[J].作物学报,2011,37(6):955-964,10.基金项目
本研究由国家自然科学基会项目(30771313)和国家科技支撑计划项目(2008BAD97B02)资助. （30771313）
