作物学报2011，Vol.37Issue(6)：975-981,7.DOI:10.3724/SP.J.1006.2011.00975
利用分子标记辅助选择聚合水稻抗病基因Pi-ta、Pi-b和Stv-bi
Pyramiding Resistance Gene Pi-ta,Pi-b,and Stv-bi by Marker-assisted Selection in Rice(Oryza sativa L.)
摘要
Abstract
The blast and stripe disease are two major diseases of rice in Yangtze River japonica cultivating area.Breeding resistant cultivars is the most effective way to prevent these two major diseases.Wuyujing 8, containing both Pi-ta and Pi-b, was crossed with Zhendao 42 which carried rice stripe disease resistance gene Stv-bi.Two gene-markers, Pita and Pib, and one SCAR marker tightly linked with Stv-bi were used for marker-assisted selection in each segregating generation.After the multi-generation breeding and identification of resistance, three resistance genes were put together finally.One new rice stable line, 74121 was obtained which conferred good agronomic characteristics containing high-yield, good quality and multi-resistance.These results indicated that marker-assisted selection could not only be a simple and effective way but also provide genetic resources for breeding new rice multi-resistant varieties.关键词
水稻/稻瘟病/条纹叶枯病/Pi-ta/Pi-b/Srv-bi/分子标记辅助选择Key words
Rice/ Blast/ Stripe disease/ Pi-ta/ Pi-b/ Stv-bi/ Marker-assisted selection引用本文复制引用
王军,杨杰,陈志德,范方军,朱金燕,杨金欢,仲维功..利用分子标记辅助选择聚合水稻抗病基因Pi-ta、Pi-b和Stv-bi[J].作物学报,2011,37(6):975-981,7.基金项目
本研究由江苏省农业科技自主创新资金项目(cx[10]402),国家科技支撑计划重大项目(2006BADO2A03),江苏省科技支撑计划项目(BE2009301-2),国家转基因生物新品种培育科技重大专项(2009ZX08001-005B,019)和江苏省自然科学基金项目(BK2009321)资助. （cx[10]402）
