作物学报2011，Vol.37Issue(6)：991-997,7.DOI:10.3724/SP.J.1006.2011.00991
水稻ygl98黄绿叶突变基因的精细定位与遗传分析
Genetic Analysis and Fine-mapping of ygl98 Yellow-green Leaf Gene in Rice
摘要
Abstract
A yellow-green leaf mutant ygl98 was isolated by chemistry mutagenesis. The whole plant of the mutant exhibited yellow-green trait throughout the growing period. Compared to its wild-type parent 10079, the contents of chlorophyll and carotenoid decreased by 45.3％ and 45.6％; and at maturity, the number of productive panicles per plant, seed setting rate and plant height reduced by 14.4％, 10.7％, and 7.4％, respectively. The result of electron microscopic observation revealed that the chloroplasts in the ygl98 mutant were out-of-shape. A lot of cystic structures and poor thylakoids were observed in the chloroplasts of the ygl98 mutant, and grana stacks appeared to be less dense compared to those of the wild type. Genetic analysis showed that the yellow-green leaf trait of the ygl98 mutant was controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant gene was conducted using 771 yellow-green leaf individuals from the F2 mapping population of ygl98/Zhefu 802. Finally, the mutant gene was mapped between InDel markers I3 and I4 on the long arm of chromosome 3, with genetic distances of 0.07 and 0.19 cM, respectively, and with physical distance of 44.2 kb, in which eight predicted genes had been annotated. Sequencing analysis of these candidate genes between the mutant and its wild-type revealed the single base change (G1522A) of the gene for magnesium-chelatase Ch1D subunit resulted in a missense mutation (A508T) in the encoded product. The same gene mutation caused by OsChlD (Chlorina-1) was documented previously. The Chlorina-1 mutant displays a severe yellowish-green leaf phenotype only at the seedling stage, and the abnormal leaf color is first observed on the leaves of 2- to 3-week-old seedlings, while the ygl98 mutant exhibits yellow-green trait throughout the growing period. The different phenotypes of the two mutants may be caused by different mutational sites of OsChlD genomic sequence.关键词
水稻/黄绿叶突变体/OsChlD/遗传分析/精细定位Key words
Oryza sativa L./ Yellow-green leaf mutant/ OsChlD/ Genetic analysis/ Fine-mapping引用本文复制引用
孙小秋,王兵,肖云华,万春美,邓晓建,王平荣..水稻ygl98黄绿叶突变基因的精细定位与遗传分析[J].作物学报,2011,37(6):991-997,7.基金项目
本研究由国家自然科学基金项目(31071402)和国家转基因生物新品种培育科技重大专项(2009ZX08009-072B)资助. （31071402）
