中国动脉硬化杂志2011，Vol.19Issue(5)：395-398,4.
氨氯地平对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞血小板源性生长因子B mRNA表达的影响
Effect of Amlodipine on PDGF-B mRNA Expression in HUVEC-12 Endothelial Cells Induced by Oxidized Low Density Lipoprotein
摘要
Abstract
Aim To study the effect of amlodipine on platelet-derived growth factor-B (PDGF-B) mRNA expression in HUVEC-12 endothelial cells induced by oxidized low density lipoprotein (ox-LDL) to explore the new antiatherosclerotic role of amlodipine.Methods HUVEC-12 endothelial cells were treated with 50 mg/L ox-LDL for 24 hours through the preexperiment.The experiment groups included 5 groups: control group, ox-LDL group, different concentration (0.1, 1.0 and 10.0μmol/L) of amlodipine pretreated for 0.5 h and treated with 50 mg/L ox-LDL for 24 h group.RT-PCR was used to detect the mRNA expression of PDGF-B.Results RT-PCR analysis showed that with ox-LDL and amlodipine treatment, the mRNA expression of PDGF-B was greatly decreased in a concentration-dependent manner.Conclusion Ox-LDL can affect the mRNA expression of PDGF-B in the HUVEC-12 endothelial cells.Amlodipinecan down-regulate the mRNA expression of PDGF-B.关键词
氨氯地平/氧化型低密度脂蛋白/人脐静脉内皮细胞/血小板源性生长因子BKey words
Amlodipine/ Oxidized Low Density Lipoprotein/ Human Umbilical Vascular Endothelial Cells/ Platelet-Derived Growth Factor-B分类
医药卫生引用本文复制引用
何谨,孟军,周志刚,周喜,涂剑,涂玉林..氨氯地平对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞血小板源性生长因子B mRNA表达的影响[J].中国动脉硬化杂志,2011,19(5):395-398,4.基金项目
湖南省医药卫生基金(B2007101)资助 （B2007101）
