中国动脉硬化杂志2011，Vol.19Issue(5)：409-412,4.
脂氧素A4抑制单核巨噬细胞源性树突状细胞的分化及成熟
Lipoxin A4 Inhibits Lipopolysaccharide-induced Differentiation of RAW264.7 Murine Macrophages into Dendritic-like Cells and Its Maturation
陈样新 1罗年桑 1王晓俏 2林永青 1聂如琼 1王景峰1
作者信息
- 1. 中山大学孙逸仙纪念医院心内科,广东省广州市,510120
- 2. 广州市妇女儿童医疗中心麻醉科,广东省广州市,510623
- 折叠
摘要
Abstract
Aim To investigate the effects of lipoxin A4 (LXA4) on lipopolysaecharide (LPS)-induced differentiation and mature of RAW264. 7 macrophages into dendritic like cells (DC). Methods RAW264. 7 murine macrophages were respectively treated with LPS (0 μg/L and 100 μg/L), LPS (100 μg/L) together with LXA4 (100 nmol/L and 500 nmol/L). Cells surface markers (CD80, CD86 and MHC-Ⅱ) were analyzed by FACS, and cell morphology was observed by laser scanning confocal microscope and phase contrast microscope. Results LPS could siguificantly promote the differentiation of RAW264. 7 murine macrophages into DC and the expression of surface molecules ( CD80, CD86,MHC-Ⅱ) (P ＜ 0. 01 ), and LXA4 could inhibit the differentiation of LPS-stimulated RAW264.7 murine macrophages and down-regulate the expression of CD86 and MHC-Ⅱ, but had no significant effect on the expression of CD80. Conclusion LXA4 could negatively regulate the differentiation of RAW264. 7 into DC and inhibit the maturation of DC, which may inhibit the pathogenesis and development of atherosclerosis by negatively regulating immuno-inflammatory response.关键词
脂氧素A4/巨噬细胞/树突状细胞/炎症/动脉粥样硬化Key words
Lipoxin A4/ Macrophages/ Dendritic Cells/ Inflammation/ Atherosclerosis分类
医药卫生引用本文复制引用
陈样新,罗年桑,王晓俏,林永青,聂如琼,王景峰..脂氧素A4抑制单核巨噬细胞源性树突状细胞的分化及成熟[J].中国动脉硬化杂志,2011,19(5):409-412,4.
