广西植物2011，Vol.31Issue(3)：418-421,4.DOI:10.3969/j.issn.1000-3142.2011.05.026
HPLC法测定牡荆中牡荆素的含量
Determination of vitexin in Vitex negundo var.cannabifolia by HPLC
摘要
Abstract
A method was established for determining contents of vitexin in Vitex negundo var. cannabifolia (Sieb. et Zucc. )by HPLC in order to evaluate this resource. Vitexin was obtained by ultrasonic extraction, separated on Filo door column(5 μm,250 mm×4. 6 mm)with a mixture of methanol- water(volume ratio 4: 6)as mobile phase at flow rate 1.0 mL · min-1. The UV detective wavelength was 340 nm,injection volume was 5 μL,and the column temperature was set at 30 ℃C. Vitexin content showed a good linear relationship within 0. 04520-0. 4068 μg. (r=0. 9998)and the average recovery was 100. 4 ％, RSD was 1.63 ％. This method is simple,accurate and can be used for quality control of Vitex negundo var. cannabi f olia.关键词
超声提取/高效液相色谱/牡荆/含量测定Key words
ultrasonic eextraction/ HPLC/ Vitex negundo var. cannabifolia/ determination分类
农业科技引用本文复制引用
罗娅君,边清泉,陈佳,蒋云福,陈驹..HPLC法测定牡荆中牡荆素的含量[J].广西植物,2011,31(3):418-421,4.基金项目
四川省教育厅重点项目(092A180) （092A180）
绵阳师范学院科研项目(MA2009017) （MA2009017）
