中国临床保健杂志2011，Vol.14Issue(3)：287-289,3.DOI:10.3969/J.issn.1672-6790.2011.03.024
虫草肾茶胶囊对人肾小球系膜细胞分泌转化生长因子β1及细胞外基质的影响
The effect of Chongcaoshencha capsule on the expression of TGF-β1and ECM of human mesangial cells in high glucose
摘要
Abstract
Objective To investigate the effect of Chongcaoshencha capsule on the expression of TGF-β1 and ECM of human mesangial cell (HMC) in vitro under high glucose. To explore the nephroprotective mechanism of Chongcaoshencha capsule for DN. Methods We first get the rats serum with different concentration of Chongcaoshencha capsule and fosinopril. Enzyme linked immunoadsordent assay (ELISA) was adopted to determine the expression of TGF-β1 ,fibronectin and laminin in cultured human mesangial cells,and the Fosinopril Sodium group was control. Results In vitro, the expressions of TGF-β1 ,fibronectin and laminin were obviously promoted under the stimulation of high glucose (P ＜ 0.05 or P ＜ 0.01 ); but Chongcaoshencha capsule can inhibit HMCs secret to TGF-β1 ,fibronectin and laminin in a dose effect manner ( P ＜ 0.05 or P ＜ 0. 01 ), and in some respect the Chongcaoshencha capsule was more effective than the Fosinopril Sodium. Conclusion High glucose can promote secretion of TGF-β1,fibronectin and laminin by HMC, but Chongcaoshencha capsule could inhibit them, which could be helpful for preventing diabetic nephropathy.关键词
糖尿病肾病/肾小球系膜细胞/转化生长因子β/细胞外基质/中草药Key words
Diabetic nephropathies/ Ransforming growth factor beta/ Extracellular matrix/ Drugs分类
医药卫生引用本文复制引用
金丽霞,宋立群,宋业旭..虫草肾茶胶囊对人肾小球系膜细胞分泌转化生长因子β1及细胞外基质的影响[J].中国临床保健杂志,2011,14(3):287-289,3.基金项目
黑龙江省教育厅科学技术研究项目(11511357) （11511357）
