解剖学杂志2011，Vol.34Issue(3)：327-330,4.
细胞外调节蛋白激酶信号通路在体外诱导神经干细胞向施万细胞分化中的作用
Effect of extracellular signal-regulated protein kinase pathway on the differentiation of rat neural stem cells into Schwann cells *
摘要
Abstract
Objective: To study the effect of ERK pathway on the differentiation of rat neural stem cells into Schwann cells.Methods: Neural stem cells (NSCs) were cultured in vitro and were induced to differentiate into Schwann cells by adding a mixture of reagents into the medium. Cells were divided into control group cultured in the induction media and experimental group cultured in the mixture of NSCs media and inhibitor of ERK pathway. S100- and P75-positive ceils were labeled by double-labelling immunofluorescence. Results: After the administration of induction reagents, the level of phosphorylatedERK1/2 was elevated; Three weeks later, S100- and P75-positive cells were observed. The level of phosphorylated-ERK1/2 in the experimental group was decreased with administration of U0126. Schwann cells in the ERK group were significantly less than the control group. Conclusion: ERK signal pathway plays an important role in the induction of NSCs into Schwann cells.关键词
神经干细胞/施万细胞/细胞外调节蛋白激酶信号通路/分化Key words
neural stem cells/ Schwann cells/ extracellular signal-regulated protein kinase pathway/ differentiation引用本文复制引用
佟雷,王振宇,季丽莉,佟晓杰,方秀斌..细胞外调节蛋白激酶信号通路在体外诱导神经干细胞向施万细胞分化中的作用[J].解剖学杂志,2011,34(3):327-330,4.基金项目
国家自然科学基金(81070011) （81070011）
辽宁省科技厅社会发展攻关计划(2010225029) （2010225029）
