南方医科大学学报2011，Vol.31Issue(6)：991-994,4.
吉非替尼对鼻咽癌细胞株CNE2放疗增敏作用
Gefitinib enhances the radiosensitivity of nasopharynheal carcinoma cell line CNE2 in vitro
摘要
Abstract
Objective To study the radiosensitizing effect of gefitinib on nasopharyngeal carcinoma cell line CNE2 in vitro. Methods Nasopharyngeal carcinoma cell line CNE2 was cultured in RP2MI1640. MIT assay was performed to evaluate the cell proliferation changes in response to gefitinib treatment and the radiosensitizing effect of gefitinib. The cell survival curves and sensitive enhancement ratio (SERs) were obtained with a clonogenic assay. Flow cytometry analysis was applied to detect the cell cycle changes and cell apoptosis. Results Mil assay showed that cells exposed to gefitinib and radiation had a significantly lower survival ratio compared to the cells with radiation exposure only (0.582 ±0.012 vs 0.398 ±0.016, P=0.002), with a SER of 1.535 ±0.134. The S phase cell percentage was significantly decreased and G2-M phase cells increased in gefitinib plus radiation group (P=0.000), suggesting a synergistic effect of gefitinib and radiation. Conclusion Gefitinib can enhance the radiosensitivity of nasopharyngeal carcinoma CNE2 cells in vitro possibly by inhibiting cell proliferation, inducing cell apoptosis, and causing changes in the cell cycle distribution.关键词
吉非替尼/放射增敏/细胞周期/细胞凋亡/鼻咽癌细胞Key words
gefitinib/ radiosensitivity/ cell cycle/ apoptosis/ nasopharyngeal carcinoma cells分类
医药卫生引用本文复制引用
何本夫,孙爱民,黄碧燕,王雯珺 ,郑小康,罗荣城..吉非替尼对鼻咽癌细胞株CNE2放疗增敏作用[J].南方医科大学学报,2011,31(6):991-994,4.基金项目
国家自然科学基金(30973846) （30973846）
广东省自然科学基金博士启动 项N(8451051501001344) （8451051501001344）
