南方医科大学学报2011，Vol.31Issue(6)：1017-1019,3.
深圳散发腹泻患者札如病毒的检测及型别分析
Detection and genotype analysis of sapovirus associated with sporadic diarrhea in Shenzhen in 2009
摘要
Abstract
Objective To conduct an epidemiological and genotype analysis of sapovirus (SaV) associated with sporadic diarrhea in Shenzhen in the year 2009. Methods A total of 852 fecal samples were collected from sporadic cases of diarrhea in Shenzhen in 2009 and detected by reverse-transcription polymerase chain reaction (RT-PCR) using the primers of SLV5317/5749. The PCR products were analyzed with 1.5% agarose gel electrophoresis and sequenced to construct the phylogenetic tree. Results Sixteen samples were found positive for SaV, with a positivity rate of 1.88%. Sequence analysis identified 8 isolates as SaV GI genotype (including 3 SaV GI.l and 5 SaV GI.2), 7 as SaV GIV genotype, and 1 as Gil genotype. Conclusion SaV infection is present in Shenzhen with GI as the predominant genotype. This is the first report of SaV GIV strains in China, which differs from the strains of Anhui-A141 and Beijing-CHN99/BJ360, suggesting the genotypic variety of SaV infection in China.关键词
札如病毒/腹泻/流行病学/基因型别Key words
sapovirus/ diarrhea/ epidemiology/ genotype分类
医药卫生引用本文复制引用
王颜歌,何雅青,向文龙,廖玉学,陈清,俞守义,胡贵方..深圳散发腹泻患者札如病毒的检测及型别分析[J].南方医科大学学报,2011,31(6):1017-1019,3.基金项目
"十一五"国家科技重大专项课题(2009ZX1001-081) （2009ZX1001-081）
