南方医科大学学报2011，Vol.31Issue(6)：1020-1022,3.
模拟微重力对单核细胞增殖及组织因子mRNA表达的影响
Effect of simulated microgravity on human monocytic cell proliferation and tissue factor mRNA expression
摘要
Abstract
Objective To investigate the effect of simulated microgravity on the proliferation of human monocytic cells THP-1 and the expression of tissue factor (TF) mRNA. Methods THP-l cells were cultured under a simulated microgravity environment using the rotating cell culture system (RCCS). The changes in the cell proliferation after microgravity culture were assessed by cell counting and cell cycle analysis with flow cytometry. RT-PCR was used to detect the changes in the expression of TF mRNA in THP-1 cells. Results Culture under simulated microgravity resulted in a significant decrease in the cell number of THP-1 cells in comparison with that of the control cells (P0.01). After a 24-h culture under microgravity, the Go-Gi phase cells increased from the control level of (46.57 ±1.64)% to (67.64±2.71)% (P<0.05). The cells in both groups showed a low level of TF mRNA expression in the absence of LPS stimulation. A 4-h stimulation with LPS caused up-regulated expression of TF mRNA in both cells, but the microgravity group showed a significantly smaller increase in the expression (2.301 ±0.179) than the control group (9.210±1.328) (P<0.05). Conclusion Microgravity can inhibit the proliferation of THP-1 cells and suppress the cellular expression of TF mRNA.关键词
微重力/单核细胞/细胞增殖/组织因子/mRNAKey words
microgravity/ monocytic cells/ cell proliferation/ tissue factor/ mRNA分类
医药卫生引用本文复制引用
于霞,郑磊,熊石龙,蔡贞,王前..模拟微重力对单核细胞增殖及组织因子mRNA表达的影响[J].南方医科大学学报,2011,31(6):1020-1022,3.基金项目
国家自然科学基金(81071416) （81071416）
国防科工委专项课题(B3320062101) （B3320062101）
广东省自然科学基金(07300312) （07300312）
南方医院院长基金(2009Z004) （2009Z004）
