南方医科大学学报2011，Vol.31Issue(6)：1086-1089,4.
肠道蛋白酶在失血性休克大鼠炎症反应中的作用
Effect of prolease in the lumen of rat intestine on inflammatory reaction during hemorrhagic shock
摘要
Abstract
Objective To investigate the effect of intraluminal administration of ulinastatin (a prolease inhibitor) in the intestine on intestinal inflammation in rats with hemorrhagic shock. Methods Twenty-eight Wistar rats were randomized into control group (A), intestinal saline perfusion group (B), ulinastatin intestinal perfusion group (C), and intravenous ulinastatin injection group (D) (n=7). The mean arterial blood pressure (MAP) and survival time of the rats were recorded. The changes in human polymorphonuclear cell (PMN) CD11b expression were detected byflow cytometry. The leukocyte count was recorded at different time points after the treatment, and the pathology of the intestinal mucosa was observed comparatively. Results Groups C and D showed significantly slower reduction of the MAP than groups A and B after hemorrhagic shock (P<0.05). The survival time of the rats was the longest in group C (P<0.05). CDllb expression increased gradually during hemorrhagic shock in all the groups, but the expression level was the lowest in group C (P<0.05). Hemorrhagic shock caused a reduction in leukocyte counts, which remained the highest in group C (P<0.05). Group C also showed the least intestinal pathology among the 4 groups. Conclusion Intestinal perfusion of ulinastatin can lower the reduction rate of MAP, attenuate plasma activation and intestinal inflammation, and prolong the survival of rats with hemorrhagic shock. These results indicate an important role of prolease in intestinal inflammation during hemorrhagic shock.关键词
肠道灌注/蛋白酶抑制剂/乌司他丁/失血性休克/血浆活性因子Key words
intestinal perfusion/ pancreatic proteases inhibition/ ulinastatin/ hemorrhagic shock/ plasma activity分类
医药卫生引用本文复制引用
郭培培,陈仲清,席小丽,陈辉,傅卫军,王瑞亭..肠道蛋白酶在失血性休克大鼠炎症反应中的作用[J].南方医科大学学报,2011,31(6):1086-1089,4.基金项目
天普研究基金(01201028) （01201028）
