中国动脉硬化杂志2011，Vol.19Issue(8)：627-631,5.
负向调控调节性T细胞对小鼠动脉粥样硬化形成的影响
Roles of Naturally Regulatory T Cells Regulated Negatively in Experimental Atherosclerosis of ApoE-/- Mice
摘要
Abstract
Aim The aim of this study was to examine the effect of naturally regulatory T cells on atherosclerosis plaque in apoliprotein (apo)E-/- mice, through negative regulating FOXP3 gene. Methods Lentivirus-mediated (siRNA) was used and Foxp3high+ CD4 + CD25 + Treg cells adoptive transfer assays in high fat diet ApoE -/- mice were done. Its number was identified by FACS. Inflammatory cytokines were determined by ELISA and the area of atherosclerosis plaque was analyzed. Result We found that the number and function of Foxp3 + CD4 + CD25 + Treg cells in mice injected with Foxp3high+ CD4 + CD25+ Treg cells was significantly high compared with those of mice injected with FOXP3-siRNA lentivirus. Conclusion Naturally regulatory T cells regulated negatively can promote significantly the progression of atherosclerosis plaque in ApoE -/- mice.关键词
天然调节性T细胞/动脉粥样硬化/FOXP3-siRNA慢病毒载体Key words
Naturally Regulatory T Cells/ Atherosclerosis/ siRNA分类
医药卫生引用本文复制引用
王治校,周建华,毛山,詹中群,何朝荣,王崇全..负向调控调节性T细胞对小鼠动脉粥样硬化形成的影响[J].中国动脉硬化杂志,2011,19(8):627-631,5.基金项目
国家自然科学基金项目(C03030201)资助 （C03030201）
